STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING


SUNGUR S., ELCIN Y., AKBULUT U.

BIOMATERIALS, cilt.13, ss.795-800, 1992 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 13 Konu: 11
  • Basım Tarihi: 1992
  • Doi Numarası: 10.1016/0142-9612(92)90020-o
  • Dergi Adı: BIOMATERIALS
  • Sayfa Sayıları: ss.795-800

Özet

Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).