Research Information System
MICROBIAL PATHOGENESIS, vol.214, 2026 (SCI-Expanded, Scopus)
Rising antimicrobial resistance necessitates alternative therapeutic approaches for bovine mastitis, especially strategies targeting biofilm-related infections. This study explores the antibiofilm effects of LAB-derived postbiotic preparations (neutralized cell-free supernatants, nCFS) from Pediococcus pentosaceus and Lactococcus lactis, isolated from local sources as potential complementary or adjuncts to conventional antibiotic therapy. Antimicrobial activity was assessed using the agar well diffusion method, while antibiofilm efficacy was determined via the microtiter plate assay. Biofilm eradication assays showed that L. lactis CNM81-derived postbiotic preparations removed up to 93% Staphylococcus aureus biofilms, with 63-94% reductions in other Gram-positive pathogens, including Streptococcus agalactiae and Staphylococcus epidermidis. In contrast, postbiotic preparations from P. pentosaceus M46 were more effective against Gram-negative pathogens, achieving up to 88% biofilm removal in Pseudomonas aeruginosa and 74% in Klebsiella pneumoniae. Several pathogen-treatment combinations exceeded the 50% eradication threshold, highlighting the condition-dependent nature of the antibiofilm activity. Whole-genome sequencing was performed to support the bioactive potential of L. lactis CNM81. Scanning electron microscopy (SEM) revealed extensive structural disruption of mature biofilms, particularly in S. aureus. Raman spectroscopy further confirmed molecular-level biofilm damage, showing marked reductions in carotenoid- and protein-associated bands following treatment with L. lactis-derived postbiotic preparations. These LAB-derived postbiotic preparations represent a promising non-antibiotic strategy against S. aureus, the most prevalent pathogen implicated in both clinical and subclinical forms of mastitis, particularly in chronically infected mammary glands.