From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues


Belder N., Coskun O., ERDOĞAN B. D. , İLK DAĞ Ö., SAVAŞ B., ENSARİ A., ...More

PATHOLOGY RESEARCH AND PRACTICE, vol.212, no.8, pp.678-685, 2016 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 212 Issue: 8
  • Publication Date: 2016
  • Doi Number: 10.1016/j.prp.2015.11.008
  • Journal Name: PATHOLOGY RESEARCH AND PRACTICE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.678-685
  • Keywords: FFPE tissues, RNA isolation, Gene expression, Microarray, PARAFFIN-EMBEDDED TISSUES, GENE-EXPRESSION ANALYSIS, OLIGONUCLEOTIDE MICROARRAYS, EXTRACTION METHODS, ARCHIVAL MATERIAL, FROZEN TISSUE, NUCLEIC-ACIDS, TUMOR SAMPLES, DEGRADED RNA, CANCER

Abstract

Background: Genome-wide gene expression profiling analysis of FFPE tissue samples is indispensable for cancer research and provides the opportunity to evaluate links between molecular and clinical information, however, working with FFPE samples is challenging due to extensive cross-linking, fragmentation and limited quantities of nucleic acid. Thus, processing of FFPE tissue samples from RNA extraction to microarray analysis still needs optimization.