From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues


Belder N., Coskun O., ERDOĞAN B. D. , İLK DAĞ Ö. , SAVAŞ B., ENSARİ A., ...Daha Fazla

PATHOLOGY RESEARCH AND PRACTICE, cilt.212, ss.678-685, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 212 Konu: 8
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.prp.2015.11.008
  • Dergi Adı: PATHOLOGY RESEARCH AND PRACTICE
  • Sayfa Sayıları: ss.678-685

Özet

Background: Genome-wide gene expression profiling analysis of FFPE tissue samples is indispensable for cancer research and provides the opportunity to evaluate links between molecular and clinical information, however, working with FFPE samples is challenging due to extensive cross-linking, fragmentation and limited quantities of nucleic acid. Thus, processing of FFPE tissue samples from RNA extraction to microarray analysis still needs optimization.