COMPARISON OF WILD TYPE AND UPTAKE HYDROGENASE DEFICIENT MUTANT STRAINS OF RHODOBACTER CAPSULATUS FOR HYDROGEN AND PHB PRODUCTION

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Tarlan E., Hoşafcı E., Bayramoğlu T. H., Koku H.

23rd World Hydrogen Energy Conference, İstanbul, Turkey, 26 - 30 June 2022, pp.83

  • Publication Type: Conference Paper / Summary Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.83
  • Middle East Technical University Affiliated: Yes

Abstract

In this study two strains of Rhodobacter capsulatus were compared in terms of efficiency of hydrogen (H2) and poly-β-hydroxybutyrate (PHB) synthesis. One strain of R. capsulatus (YO3 (hup-)) lacked the uptake hydrogenase enzyme, while the other was its wild type (WT). Both strains were grown in a medium with high carbon and minimal nitrogen. The H2 and PHB contents of the reactors were all measured at the end of the experiment. The cell concentration measurements show that the growth trends were similar in both strains. The maximum H2 productivities of the YO3 (hup-) strain and WT strain were found as 0.534 mmol/(L.h) and 0.323 mmol/(L.h), respectively. While the PHB amount of the YO3 (hup-) strain was 11.7%, the PHB amount of the WT strain was 10.1%. More studies will be conducted to further optimize the H2 and PHB production, and to understand the partition of resources towards H2 and PHB.