Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(alpha-amino acids)


Sedlacik T., Acar O. K. , Studenovska H., Kotelnikov I., Kucka J., Konecna Z., ...Daha Fazla

SOFT MATTER, cilt.14, ss.228-238, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 14 Konu: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1039/c7sm02074k
  • Dergi Adı: SOFT MATTER
  • Sayfa Sayıları: ss.228-238

Özet

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(alpha-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N-5-(2-hydroxyethyl)-L-glutamine-stat-N-5-(2-methacryloyl-oxy-ethyl)- L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cell-supporting moieties (RGDS peptides) by the azide-alkyne "click"-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.