Electrochemical catechol biosensor based on beta-cyclodextrin capped gold nanoparticles and inhibition effect of ibuprofen

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Kapan B., Kurbanoglu S., Esenturk E., Soylemez S., TOPPARE L. K.

PROCESS BIOCHEMISTRY, cilt.108, ss.80-89, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 108
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.procbio.2021.06.004
  • Dergi Adı: PROCESS BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.80-89
  • Anahtar Kelimeler: Catechol detection, Enzyme inhibition, beta cyclodextrin, Gold nanoparticles, Ibuprofen, Tyrosinase, ENZYME-INHIBITION, SILVER NANOPARTICLES, IMMOBILIZATION, GRAPHENE, EQUATION, SENSOR, FOOD
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Herein, beta-cyclodextrin-capped gold nanoparticle surface designed for tymsinase (Tyr)-based nanosensor is proposed and demonstrated. Integration of the beta-cyclodextrin-capped gold nanoparticles on graphite electrode surface was achieved via drop-casting method, Tyr was immobilized on the modified electrode and then used as drug inhibition platform with catechol as the substrate. All optimization studies that affect biosensor response were conducted, and catechol was detected in the linear range of 1.56 mu M-25 mu M catechol concentration with a limit of detection of 0.42 mu M and sensitivity of 2.094 mu A.mu M-1.cm(-2).Tyr inhibition was followed with ibuprofen drug active compound with a 15 min incubation time, and the I-50 value was found as 213 mu M. The sensor is the first in the literature to use an electrochemical method for beta-cyclodextrin-capped gold nanoparticles based Tyr biosensor and ibuprofen inhibition. This sensor presents an easy fabrication method, excellent sensor properties, and ibuprofen inhibition capabilities. As a result, it is proposed that the designed sensor is an ideal marker for detecting Tyr inhibition using electrochemical methods and an ibuprofen-based cosmetic cream formulation could be a viable option in the cosmetic market.