Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients


Eren E., Aytac U., Tetik E., Akman O., Kansu E., Gunduz U.

AMERICAN JOURNAL OF HEMATOLOGY, vol.63, no.2, pp.85-89, 2000 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 63 Issue: 2
  • Publication Date: 2000
  • Title of Journal : AMERICAN JOURNAL OF HEMATOLOGY
  • Page Numbers: pp.85-89

Abstract

Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph), However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment. Am. J. Hematol, 63:85-89, 2000, (C) 2000 Wiley-Liss, Inc.