Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients
AMERICAN JOURNAL OF HEMATOLOGY, vol.63, no.2, pp.85-89, 2000 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 63 Issue: 2
- Publication Date: 2000
- Journal Name: AMERICAN JOURNAL OF HEMATOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.85-89
- Middle East Technical University Affiliated: Yes
Abstract
Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph), However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment. Am. J. Hematol, 63:85-89, 2000, (C) 2000 Wiley-Liss, Inc.