IMMOBILIZATION OF NADH-CYTOCHROME B5 REDUCTASE INTO GELATIN BY CROSS-LINKING


YILDIRIM O. , AKBULUT U. , ARINC E., SUNGUR S.

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, ss.19-28, 1994 (SCI İndekslerine Giren Dergi) identifier

  • Basım Tarihi: 1994
  • Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
  • Sayfa Sayıları: ss.19-28

Özet

In this work NADH-cytochrome b5 reductase enzyme (EC 1.6.2.2) obtained from rabbit liver microsomes was immobilized into gelatin by crosslinking. Chromium (III) acetate and chromium (III) sulfate were used as crosslinkers. To find the most suitable immobilization parameters; effect of crosslinker, enzyme and gelatin concentrations on immobilized enzyme activity were investigated. Unbounded enzyme was determined by washing experiments.