Structural and Biological Analysis of Mesoporous Lanthanum Doped beta TCP For Potential Use as Bone Graft Material

Motameni A., DALGIÇ A. D. , ALSHEMARY A. Z. G. , KESKİN D. , EVİS Z.

MATERIALS TODAY COMMUNICATIONS, vol.23, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23
  • Publication Date: 2020
  • Doi Number: 10.1016/j.mtcomm.2020.101151
  • Keywords: beta-Tricalcium phosphate, Lanthanum, Mesoporous particles, In vitro degradation, Bone graft materials, CALCIUM-PHOSPHATE CERAMICS, TRICALCIUM PHOSPHATE, PHASE-TRANSFORMATION, THERMAL-BEHAVIOR, CARBONATE, MECHANISMS, CELLS, IONS, ZINC, DIFFERENTIATION


In this study, mesoporous particles of beta-tricalcium phosphate (beta TCP, beta Ca-3(PO4)(2)) and lanthanum (La) doped beta TCP were synthesized using wet precipitation method attached with microwave refluxing system. The obtained materials were characterized and analysed using different sort of techniques such; X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Inductively coupled plasma optical emission spectroscopy (ICP-OES), Brunauer-Emmett-Teller (BET) and helium pycnometer. With incorporation of La3+ ions, relevant expansion in lattice parameters of beta TCP crystal was observed along with inhibition growth rate was observed. beta TCP and La-beta TCP materials were mesoporous in nature, pore diameter and pore volume were expanded with incorporation of La3+ ions. The loading/release on beta TCP and La-beta TCP particles surfaces were evaluated using fetal bovine serum (FBS) proteins. In vitro cell culture studies were performed with human osteosarcoma cell line (Saos-2). The outcomes approved that all La-beta TCP materials were cytocompatible and strict dose dependent effect of La3+ ions was observed on cell viability and alkaline phosphatase (ALP) activity. These results strongly suggest that La-beta TCP materials have a potential application in bone tissue engineering.