Effect of chromium salts on invertase immobilization onto carboxymethylcellulose-gelatine carrier system

Emregul E., Sungur S., Akbulut U.

BIOMATERIALS, cilt.17, sa.14, ss.1423-1427, 1996 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 14
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1016/0142-9612(96)87285-1
  • Dergi Adı: BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1423-1427
  • Anahtar Kelimeler: gelatine, carboxymethylcellulose, invertase enzyme, immobilization
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

The carboxymethylcellulose-gelatine carrier system was investigated for invertase immobilization. Chromium (III) acetate, chromium (III) sulphate and potassium chromium (III) sulphate were used as cross-linking agents. Effect of carboxymethylcellulose-gelatine ratio and cross-linker concentration on immobilized enzyme activity were analysed. Reusability of immobilized enzyme was also investigated. Maximum immobilized enzyme activities were obtained with cross-linkers chromium (III) sulphate (0.004 mol dm(-3)) and potassium chromium (III) sulphate (0.001 mol dm(-3)) for a carrier composition of carboxymethylcellulose-gelatine ratio 0.111 (w/w) as 78%. (C) 1996 Elsevier Science Limited