A new amperometric cholesterol biosensor based on poly(3,4-ethylenedioxypyrrole)


Turkarslan O., Kayahan S. K. , TOPPARE L. K.

SENSORS AND ACTUATORS B-CHEMICAL, vol.136, no.2, pp.484-488, 2009 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 136 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1016/j.snb.2008.10.016
  • Journal Name: SENSORS AND ACTUATORS B-CHEMICAL
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.484-488
  • Keywords: Amperometric biosensor, Enzyme electrodes, Cholesterol, Cholesterol oxidase, Conducting polymers, PEDOP, CONDUCTING POLYMERS, GLUCOSE-OXIDASE, ENTRAPMENT, LACTATE, FILM

Abstract

Cholesterol oxidase (ChOx) was physically entrapped in poly(3,4-ethylenedioxypyrrole) (PEDOP) to construct an amperometric cholesterol biosensor. The responses of the enzyme electrodes were measured via monitoring oxidation current of H2O2 at +0.7V in the absence of a mediator. Kinetic parameters, operational and storage stabilities, pH and temperature dependencies were determined. K-m, I-max and sensitivity (I-max/K-m) were calculated as 3.4 mM, 34 mu A cm(-2) and 10 mu A mM(-1) cm(-2), respectively. The minimum detectable substrate concentration was 0.4 mM and for a period of 20 days the biosensor showed the maximum relative activity. (C) 2008 Elsevier B.V. All rights reserved.