Multifunctional periodontal membrane for treatment and regeneration purposes

Isik G., HASIRCI N., TEZCANER A., KIZILTAY A.

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, cilt.35, sa.2, ss.117-138, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1177/0883911520911659
  • Dergi Adı: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, Communication Abstracts, Compendex, EMBASE, INSPEC, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.117-138
  • Anahtar Kelimeler: Methoxy poly(ethylene glycol)-block-polycaprolactone micelle, alginate, gelatin, vitamin E, doxycycline, periodontitis, BLOCK-COPOLYMERS, ALPHA-TOCOPHEROL, VITAMIN-E, POLYMERIC MICELLES, IN-VITRO, DELIVERY, NANOPARTICLES, PEG, TISSUE, CYTOTOXICITY
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Periodontitis is a chronic inflammatory disease that causes gum tissue degeneration and alveolar bone and tooth loss. The aim of this study is to develop a multifunctional matrix for the treatment of periodontitis and enhancement of regeneration of the periodontal tissue. The matrix was prepared from vitamin E containing hydrogel made of alginate and gelatin, and doxycycline HCl containing methoxy poly(ethylene glycol)-block-polycaprolactone micelles. Methoxy poly(ethylene glycol)-block-polycaprolactone was synthesized with ring-opening polymerization technique and characterized by proton nuclear magnetic resonance (H-1 NMR), Fourier-transform infrared spectroscopy, differential scanning calorimetry, and gel permeation chromatography. Micelles were characterized by measuring zeta potential, hydrodynamic diameter, drug encapsulation efficiency, drug loading capacity, and in vitro drug-release kinetics. Micelles were obtained with an average size of 164 nm and drug loading amount of 5.8%. The activity of doxycycline HCl-loaded micelles and vitamin E containing hydrogels was determined against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) with disk diffusion method. Bio-efficacy of micelle-loaded alginate-gelatin hydrogels were tested in vitro using L929 fibroblasts and dental pulp stem cells. Doxycycline HCl-loaded micelles and vitamin E containing hydrogels showed a sustained release and exhibited inhibition zone against E. coli and S. aureus. Hydrogels with vitamin E and doxycycline HCl-loaded micelles promoted osteogenic differentiation of dental pulp stem cells. Results suggest that alginate-gelatin hydrogels containing doxycycline HCl-loaded micelles and vitamin E can be good candidates for the treatment of periodontitis and tissue regeneration.