Eriochrome Black T-Eu3+ Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores

Yilmaz M. D., ÖKTEM H. A.

ANALYTICAL CHEMISTRY, vol.90, no.6, pp.4221-4225, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 90 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.1021/acs.analchem.8b00576
  • Journal Name: ANALYTICAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.4221-4225
  • Middle East Technical University Affiliated: Yes

Abstract

A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT) Eu3+ complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV vis titration experiments demonstrated that EBT and Eu3+ ions formed a 1:1 coordination pair in water. In the presence of Eu3+ ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characteristic fluorescence emission from DPA-Eu3+ complex was observed. In addition, the sensitivity of the system was further evaluated on Geobacillus stearothermophilus spores and as low as 2.5 X 10(5) spores were detected.