Eriochrome Black T-Eu3+ Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores

Yilmaz M. D. , ÖKTEM H. A.

ANALYTICAL CHEMISTRY, cilt.90, ss.4221-4225, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 90 Konu: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1021/acs.analchem.8b00576
  • Sayfa Sayıları: ss.4221-4225


A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT) Eu3+ complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV vis titration experiments demonstrated that EBT and Eu3+ ions formed a 1:1 coordination pair in water. In the presence of Eu3+ ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characteristic fluorescence emission from DPA-Eu3+ complex was observed. In addition, the sensitivity of the system was further evaluated on Geobacillus stearothermophilus spores and as low as 2.5 X 10(5) spores were detected.