Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C


Demir G. C. , Erdemli Ö., KESKİN D. , TEZCANER A.

INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol.614, 2022 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 614
  • Publication Date: 2022
  • Doi Number: 10.1016/j.ijpharm.2021.121436
  • Title of Journal : INTERNATIONAL JOURNAL OF PHARMACEUTICS
  • Keywords: Xanthan, Gelatin, Keratin, Glycerol, Wound dressing, CROSS-LINKING, DRUG-RELEASE, MECHANICAL-PROPERTIES, HYDROGELS, SKIN, CELLULOSE, CHITOSAN, COLLAGEN, FILM, OPTIMIZATION

Abstract

Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings. This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC). Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycerol concentrations and characterized to determine the hydrogel composition. According to their weight ratios, xanthan, gelatin, and glycerol hydrogels are named. If their weight ratio is 1:1:2 (w/w/w), the group name is selected as X1:GEL1:GLY2. X1: GEL1:GLY2 hydrogel was selected for biocompatibility, mechanical property, water vapor transmission rate (WVTR), and porosity. The addition of keratin to X1:GEL1:GLY2 improved L929 fibroblasts viability and increased protein release. Water vapor transmission of XGHs and KXGHs was between 3059.09 & PLUSMN; 126 and 4523 & PLUSMN; 133 g m(-2) d(-1); therefore, they can be suitable for granulating, low to moderate exudate wounds. XGH and KXGHs loaded with VC had higher water uptake, making it more convenient for exudate wounds. VC was released for 100 h, and VC containing XGHs and KXGHs increased the collagen synthesis of L929 fibroblasts. All of the hydrogels (XGH, KXGH, and VC-KXGHs) inhibited the bacteria transmission. In conclusion, our results suggest that VC-XGH and VC-KXGH can be candidates for temporary wound dressing materials for skin wounds.