Effect of double growth factor release on cartilage tissue engineering

Creative Commons License

Ertan A. B. , Yilgor P., Bayyurt B. , Calikoglu A. C. , Kaspar C., Kök F. N. , ...More

Journal of Tissue Engineering and Regenerative Medicine, vol.7, no.2, pp.149-160, 2013 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 2
  • Publication Date: 2013
  • Doi Number: 10.1002/term.509
  • Title of Journal : Journal of Tissue Engineering and Regenerative Medicine
  • Page Numbers: pp.149-160
  • Keywords: Cartilage tissue engineering, Cell differentiation, Growth factors, Mesenchymal stem cells, Peptide and protein delivery


The effects of double release of insulin-like growth factor I (IGF-I) and growth factor β1 (TGF-β1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF-β1, respectively. On tissue culture polystyrene (TCPS), TGF-β1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200-400μm) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-β1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue. © 2011 John Wiley & Sons, Ltd.