Biochemistry, genetics and regulation of bacilysin biosynthesis and its significance more than an antibiotic


NEW BIOTECHNOLOGY, vol.32, no.6, pp.612-619, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 6
  • Publication Date: 2015
  • Doi Number: 10.1016/j.nbt.2015.01.006
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.612-619
  • Middle East Technical University Affiliated: Yes


Bacillus subtilis has the capacity to produce more than two dozen bioactive compounds with an amazing variety of chemical structures. Among them, bacilysin is a non-ribosomally synthesized dipeptide antibiotic consisting of L-alanine residue at the N terminus and a non-proteinogenic amino acid, L-anticapsin, at the C terminus. In spite of its simple structure, it is active against a wide range of bacteria and fungi. As a potent antimicrobial agent, we briefly review the biochemistry and genetics as well as the regulation of bacilysin biosynthesis within the frame of peptide pheromones-based control of secondary activities. Biological functions of bacilysin in the producer B. subtilis beyond its antimicrobial activity as well as potential biotechnological use of the biosynthetic enzyme L-amino acid ligase (Lal) are also discussed.