Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate

Moral C. K., Doğan Ö., Sanin F. D.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, cilt.176, ss.875-891, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 176
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1007/s12010-015-1617-z
  • Dergi Adı: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.875-891
  • Anahtar Kelimeler: Alginate, Calcium concentration, Dissolved oxygen tension, Monomer distribution, Sucrose concentration, AZOTOBACTER-VINELANDII, CONTINUOUS CULTURES, MOLECULAR-WEIGHT, AGITATION SPEED, BATCH CULTURE, BIOSYNTHESIS, CALCIUM, GROWTH
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers. It is produced by algae and some species of Azotobacter and Pseudomonas. This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCCA (R) 9046 in a fermenter. Results showed that alginate production increased with increasing DOT from 1 to 5 %. The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT. At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration. On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).