Understanding the cell behavior on nano-/micro-patterned surfaces

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HASIRCI V. N., Pepe-Mooney B. J.

NANOMEDICINE, vol.7, no.9, pp.1375-1389, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 9
  • Publication Date: 2012
  • Doi Number: 10.2217/nnm.12.7
  • Journal Name: NANOMEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1375-1389
  • Keywords: micropatterning, nanopatterning, surface modification, tissue engineering, PIGMENT EPITHELIAL-CELLS, MECHANICAL-PROPERTIES, KERATOCYTES
  • Middle East Technical University Affiliated: Yes


Aim: This article reports on studies conducted in the same laboratory on interactions between patterned substrates with different pattern dimensions and chemistries, and various types of cells. Materials & methods: In order to compare the influence of various parameters, bone marrow stromal cells, retinal pigment epithelial cells, human corneal stromal cells (keratocytes), Saos-2 (human osteosarcoma cells), human microvascular endothelial cells and vascular smooth muscle cells were tested on surfaces with different physical patterns and chemical properties. Results: It was observed that cell type and surface topography are more influential than surface chemistry in determining the alignment tendency of a cell on a substrate surface. Low walls (several microns high) could not confine cells into the microgrooves of the films but alignment was still possible if the cells had a natural alignment property. Conclusion: This information is very useful in designing tissue engineering scaffolds and in the long-term success of implants.