This work is the first part of a global study about the CO2 mass balance in a tobacco industry, including the CO2 sequestration in the tobacco plants,CO2 sequestration in an eucalyptus forest, solar irradiation, and the approach methodology to obtain the CO2 emission of the process in the industry. The aim of this work is to identify the variations in the CO2 sequestration through the growing of tobacco plants in Chile and consequently in many parts of the world. It is necessary to correlate the on-site measured values through the tobacco plants growing with solar radiation, estimating the monthly absorbed CO2 mass from the environment. With this correlation, it is possible to obtain a CO2 balance in the industry knowing the total annual amount of emitted CO2 and to compare it with the absorbed amount by the plant. It has obtained a methodology that can be used as a guide for future researchers of carbon balances.