PURIFICATION AND CHARACTERIZATION OF HUMAN MENINGIOMA M2-TYPE PYRUVATE-KINASE
CLINICAL BIOCHEMISTRY, vol.26, no.5, pp.383-388, 1993 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 26 Issue: 5
- Publication Date: 1993
- Doi Number: 10.1016/0009-9120(93)90114-l
- Journal Name: CLINICAL BIOCHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.383-388
- Middle East Technical University Affiliated: Yes
Abstract
The M2-type pyruvate kinase was purified from human meningioma by ammonium sulfate precipitation, followed by ion exchange and affinity chromatography. The specific activity of the purified enzyme was 33.4 U/mg with a yield of 6.5%.