PURIFICATION AND CHARACTERIZATION OF HUMAN MENINGIOMA M2-TYPE PYRUVATE-KINASE

MELLATI A., YUCEL M., ALTINORS N., GUNDUZ U.

CLINICAL BIOCHEMISTRY, vol.26, no.5, pp.383-388, 1993 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 5
  • Publication Date: 1993
  • Doi Number: 10.1016/0009-9120(93)90114-l
  • Journal Name: CLINICAL BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.383-388
  • Middle East Technical University Affiliated: Yes

Abstract

The M2-type pyruvate kinase was purified from human meningioma by ammonium sulfate precipitation, followed by ion exchange and affinity chromatography. The specific activity of the purified enzyme was 33.4 U/mg with a yield of 6.5%.