Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.207, 2024 (SCI-Expanded)
The development of laccase-based biosensors, in particular, have attracted much interest due to their ability to detect highly toxic molecules in the environment. This article reports a novel biosensor for catechol detection that can be used to determine toxic molecules in the environment. For this purpose, a novel conjugated polymer (P-PBF) and conjugated polymer nanoparticles (P-PBFNP) were synthesized and characterized, respectively. To our knowledge, research has yet to be done on conjugated polymer nanoparticle-based electrochemical biosensing platforms for catechol determination. The biosensor comprises a GE/P-PBF NP/Lac electrode, constructed with P-PBF NP deposited onto a bare graphite electrode followed by laccase (Lac) enzyme immobilization. While electrochemical characterization was performed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), surface characterization was performed by field emission scanning electron microscopy (FE-SEM). The biosensor exhibited high sensitivity (1.229 μA/μM.cm2), a good linear range (0.5–25 μM) and a low limit of detection (LOD) (0.49 μM). Moreover, the developed biosensor exhibited high selectivity in the presence of interfering analytes without any memory effects. The potential of the GE/P-PBF NP/Lac electrode in tap water, green tea, synthetic urine and serum samples indicates the inspiring biosensing applications for monitoring environmental pollution.