Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review


Ozturk S., ÇALIK P., Ozdamar T. H.

TRENDS IN BIOTECHNOLOGY, vol.34, no.4, pp.329-345, 2016 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 34 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.1016/j.tibtech.2015.12.008
  • Journal Name: TRENDS IN BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.329-345

Abstract

Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due to the different intracellular production patterns, no consensus exists on the FBO strategy that gives the maximum productivity, whereas for r-protein production appropriate feeding strategies vary depending on the promoter used. Thus, we conclude that the B. subtilis community is still seeking an approved strong promoter and generalized FBO strategies.