Fed-Batch Biomolecule Production by Bacillus subtilis: A State of the Art Review

Ozturk S., ÇALIK P., Ozdamar T. H.

TRENDS IN BIOTECHNOLOGY, cilt.34, sa.4, ss.329-345, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 34 Sayı: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.tibtech.2015.12.008
  • Dergi Adı: TRENDS IN BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.329-345
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Bacillus subtilis is a highly promising production system for various biomolecules. This review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the approaches to design the initial production medium and/or feed stream. Additionally, the feeding strategies developed with or without feedback control for fed-batch B. subtilis fermentations were compiled with a special emphasis on recombinant protein (r-protein) production. For biomolecule production by wild-type B. subtilis, due to the different intracellular production patterns, no consensus exists on the FBO strategy that gives the maximum productivity, whereas for r-protein production appropriate feeding strategies vary depending on the promoter used. Thus, we conclude that the B. subtilis community is still seeking an approved strong promoter and generalized FBO strategies.