Immobilization of polyphenol oxidase in conducting copolymers and determination of phenolic compounds in wines with enzyme electrodes

Kiralp S., Toppare L., Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.33, ss.37-41, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0141-8130(03)00062-x
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.37-41
  • Anahtar Kelimeler: polyphenol oxidase, immobilization, electrochemistry, phenolic determination, wine, SPECTROPHOTOMETRIC METHOD, LIQUID-CHROMATOGRAPHY, HYDRAZONE
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Polyphenol oxidase (PPO) was immobilized in copolymers of thiophene functionalized menthyl monomer (MM) with pyrrole. Immobilization of enzyme was performed via entrapment in conducting copolymers during electrochemical polymerization of pyrrole. Maximum reaction rates. Michaelis-Menten constants and temperature, pH and operational stabilities of enzyme electrodes were investigated. Total amount of phenolic compounds in red wines of Turkey were analyzed by using these electrodes. (C) 2003 Elsevier B.V. All rights reserved.