Immobilization of polyphenol oxidase in conducting copolymers and determination of phenolic compounds in wines with enzyme electrodes


Kiralp S., Toppare L. , Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.33, pp.37-41, 2003 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33
  • Publication Date: 2003
  • Doi Number: 10.1016/s0141-8130(03)00062-x
  • Title of Journal : INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Page Numbers: pp.37-41

Abstract

Polyphenol oxidase (PPO) was immobilized in copolymers of thiophene functionalized menthyl monomer (MM) with pyrrole. Immobilization of enzyme was performed via entrapment in conducting copolymers during electrochemical polymerization of pyrrole. Maximum reaction rates. Michaelis-Menten constants and temperature, pH and operational stabilities of enzyme electrodes were investigated. Total amount of phenolic compounds in red wines of Turkey were analyzed by using these electrodes. (C) 2003 Elsevier B.V. All rights reserved.