Akademik Veri Yönetim Sistemi
ChemistryOpen, cilt.15, sa.5, 2026 (SCI-Expanded, Scopus)
In this present study, an enzyme-based amperometric nanobiosensor was designed and fabricated through the immobilization of tyrosinase onto a selenium-bearing conducting polymer (poly[BDT-alt-(TP;BSe)]), in combination with NH2-functionalized graphene quantum dots incorporating benzoselenadiazole, thienopyrroledione, and benzodithiophene moieties. The innovative nanobiosensor was developed by crosslinking the tyrosinase enzyme with the help of glutaraldehyde in a novel selenium-bearing conducting polymer and NH2 functionalized quantum dots matrices. Various factors influencing the biosensor's performance were optimized, including the amount of NH2-functionalized quantum dots, poly[BDT-alt-(TP;BSe)], tyrosinase, and glutaraldehyde. Under optimized experimental parameters, catechol detection was achieved across 0.1–88 µM with a detection limit of 0.023 µM. Subsequently, the designed biosensor is used to follow tyrosinase inhibition via rosmarinic acid-containing plant materials, specifically Rosmarinus officinalis. After optimization of the inhibition conditions, I50 values were determined as 21 µM for Rosmarinus officinalis. This represents the first literature report utilizing electrochemical methodology with a novel conducting polymer coupled with NH2-functionalized graphene quantum dots for tyrosinase biosensing to evaluate rosmarinic acid inhibitory effects.