The biochemical values serum albumin, total cholesterol, magnesium (Mg), calcium (Ca), inorganic phosphorus (Pi), sodium (Na). potassium (K) in serum (nl = 38) and zinc (Zn) and copper (Cu) in plasma (n2 = 74) were determined in individuals of Anatolian shepherd dog (Asd) which is the most preferred dog in Anatolia as a guard dog of flocks. Individuals from both sexes were collected from three different farms (sites). Comparative studies showed that (i) there were no significant differences between the values of the two sexes except for Ca, (ii) there were significant (P < 0.05-0.001) differences between the values of sites in Pi, Na. Zn and Cu. When the results of the present study was considered together with the previous ones carried on other breeds or on dogs generally, it was observed Na in Asd is in the lower limit of the observed range.