Hydrogen gas production by combined systems of Rhodobacter sphaeroides OU001 and Halobacterium salinarum in a photobioreactor

Zabut B., EI-Kahlout K., Yucel M., Gunduz U., Turker L., Eroglu I.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, cilt.31, sa.11, ss.1553-1562, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 11
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.ijhydene.2006.06.023
  • Dergi Adı: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1553-1562
  • Anahtar Kelimeler: Rhodobacter sphaeroides, Halobacterium salinarum, biohydrogen, bacteriorhodopsin, photobioreactor, combined systems, HALOBIUM, PHOTOPRODUCTION, BACTERIORHODOPSIN, BACTERIA, KINETICS, WATER
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Rhodobacter sphaeroides O.U.001 is a photosynthetic non-sulfur bacterium which produces hydrogen from organic compounds under anaerobic conditions. Halobacterium salinarum is an archaeon and lives under extremely halophilic conditions (4 M NaCl). H. salinarum contains a retinal protein bacteriorhodopsin in its purple membrane which acts as a light-driven proton pump. In this study the Rhodobacter sphaeroides O.U.001 culture was combined with different amounts of packed cells of H. salinarum S9 or isolated purple membrane fragments in order to increase the photofermentative hydrogen gas production. The packed cells of H. salinarum have the ability to pump protons upon illumination due to the presence of bacteriorhodopsin. The proton gradient produced may be used for the formation of ATP or protons may be used for H-2 production by R. sphaeroides. Similar to intact cells purple membrane fragments may also form vesicles around certain ions and may act like closed systems.