Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars

Dudchenko O. Y., Pyeshkova V. M., Soldatkin O. O., AKATA KURÇ B., Kasap B. O., Soldatkin A. P., ...Daha Fazla

NANOSCALE RESEARCH LETTERS, cilt.11, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1186/s11671-016-1275-2
  • Dergi Adı: NANOSCALE RESEARCH LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Enzyme immobilization, Silicalite, Glucose oxidase, Conductometric transducer, Biosensor, FIELD-EFFECT TRANSISTORS, IMMOBILIZED UREASE, ZEOLITES
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

The application of silicalite for improvement of enzyme adsorption on new stainless steel electrodes is reported. Glucose oxidase (GOx) was immobilized by two methods: cross-linking by glutaraldehyde (GOx-GA) and cross-linking by glutaraldehyde along with GOx adsorption on silicalite-modified electrode (SME) (GOx-SME-GA). The GOx-SME-GA biosensors were characterized by a four-to fivefold higher sensitivity than GOx-GA biosensor. It was concluded that silicalite together with GA sufficiently enhances enzyme adhesion on stainless steel electrodes. The developed GOx-SME-GA biosensors were characterized by good reproducibility of biosensor preparation (relative standard deviation (RSD)-18 %), improved signal reproducibility (RSD of glucose determination was 7 %), and good storage stability (29 % loss of activity after 18-day storage). A series of fruit juices and nectars was analyzed using GOx-SME-GA biosensor for determination of glucose concentration. The obtained results showed good correlation with the data of high-performance liquid chromatography (HPLC) (R = 0.99).