DNA fingerprinting and genetic characterization of Anatolian Triticum spp. using AFLP markers

Yildirim F., Akkaya M. S.

GENETIC RESOURCES AND CROP EVOLUTION, cilt.53, sa.5, ss.1033-1042, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 5
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1007/s10722-004-7938-3
  • Dergi Adı: GENETIC RESOURCES AND CROP EVOLUTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1033-1042
  • Anahtar Kelimeler: AFLP, DNA fingerprinting, genetic relationship and variation, Triticum spp., RESTRICTION-FRAGMENT-LENGTH, B-GENOME, WHEAT DOMESTICATION, BREAD WHEAT, IDENTIFICATION, POLYMORPHISM, PHYLOGENY, PLOIDY, ORIGIN, NUMBER
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

In this study, genetic analysis of Triticum spp. was carried out using AFLP markers. Six AFLP selective combinations were scored as presence and absence of bands for all the individual samples obtained from a single seed of each accession (70 accessions); T. baeoticum (21), T. monococcum (5), T. urartu (16), T. araraticum (7), T. dicoccoides (16) and T. dicoccon (5), resulting in 506 polymorphic AFLP bands. The phylogenetic tree showed two major clusters; one was composed of T. monococcum (AA) and T. baeoticum (AA), and the other cluster included T. araraticum (AAGG), T. dicoccon (AABB), T. dicoccoides (AABB), and T. urartu (AA). T. urartu, although having a diploid AA genome, did not cluster with other A genome diploids such as T. monococcum and T. baeoticum; instead it clustered together with the tetraploid species, confirming that T. urartu is the A genome progenitor. The extent of variations within and among species is discussed.