Cloning and heterologous expression of the extracellular alpha-galactosidase from Aspergillus fumigatus in Aspergillus sojae under the control of gpdA promoter

Gurkok S., Soyler B., Biely P., Ogel Z. B.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, vol.64, no.3-4, pp.146-149, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 3-4
  • Publication Date: 2010
  • Doi Number: 10.1016/j.molcatb.2009.09.012
  • Journal Name: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.146-149
  • Keywords: Alpha-galactosidase, Aspergillus, Heterologous expression, gpdA promoter, PURIFICATION, FUNGUS, GUAR
  • Middle East Technical University Affiliated: Yes

Abstract

Aspergillus fumigatus is highly pathogenic especially for immunocompromised people however it can efficiently produce many industrially important enzymes. The gene coding et-galactosidase enzyme (aglB) of A. fumigatus IMI 385708 has been cloned onto pAN52-4 fungal expression vector and expressed in a GRAS organism, Aspergillus sojae ATCC11906 under the control of constitutive glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. pAN52-4 fungal expression system allowed high level alpha-galactosidase production in media with simple sugar glucose as the sole carbon source and without a requirement for an inducer with a yield of 2.45 U/ml which is nearly 3-fold higher than the yield obtained from A. fumigatus grown in locust bean gum containing medium. (C) 2009 Elsevier B.V. All rights reserved.