B6-MSM Consomic Mouse Strains Reveal Multiple Loci for Genetic Variation in Sucrose Octaacetate Aversion

Ishii A., Koide T., Takahashi A., Shiroishi T., Hettinger T. P., Frank M. E., ...Daha Fazla

BEHAVIOR GENETICS, cilt.41, sa.5, ss.716-723, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 5
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s10519-011-9464-3
  • Dergi Adı: BEHAVIOR GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Social Sciences Citation Index (SSCI), Scopus
  • Sayfa Sayıları: ss.716-723
  • Anahtar Kelimeler: Sucrose octaacetate, MSM, Consomic strains, Tas2r genes, M. m. Molossinus, Soa, BITTER TASTE, GUSTATORY SENSITIVITY, SOA, C57BL/6J, DISSECTION, RECEPTORS, GUSTDUCIN, FAMILIES, SWEET
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

Based on crosses among inbred strains derived principally from M. m. domesticus, sucrose octaacetate (SOA) aversion in laboratory mice has been thought for many years to be controlled by a single genetic locus (Soa) located on distal chromosome (Chr) 6. To expand knowledge of the genetic basis underlying SOA aversion, we have studied the M. m. molossinus derived strain (MSM) and MSM consomic strains on a M. m. domesticus (C57BL/6J: B6) host background. Using two-bottle preference procedures, MSM mice avoided 0.1 mM and 1 mM SOA while B6 mice were indifferent to 0.1 mM and exhibited slight aversion to 1 mM SOA. Preference tests of 16 available consomic strains implicated Chr 2, 4 and 15 in SOA aversion in addition to the prominent effect of the known Soa locus on Chr 6 (implicated by study of two congenic strains). The originally defined Soa locus is presumably associated with one or more members of the cluster of Tas2r genes on distal Chr 6 that code for bitter taste receptors. Our results point to the possible role of established Tas2r genes on Chr 2 and 15, as well as to genes not coding for bitter receptors (Chr 4), in SOA aversion. SOA aversion emerges from this consomic screen as being definitively under polygenic control. The genetic diversity captured by the MSM model system is shown to be a valuable tool to complement the limited genetic variation in the commonly used stocks derived from M m. domesticus.