Blood transfusion is a fundamental and a requisite part of any nation's health care delivery system for life saving interventions. The study was conducted to assess knowledge, attitude, and practice and associated factors that hinder blood donation practices among the residents in Ilu Aba Bor and Buno Bedelle Zones. The study was determined level of knowledge, attitude and identified practice among residents Zones. Also factors associated with knowledge, attitude and practice of blood donation was identified. Community based survey study was conducted from March, 2017 to June, 2017. Sample size was 809. Data was collected by questionnaires tool and analyzed by descriptive and binary logistic regression. The present study showed that the prevalence of practice of blood donation were too low 24.1%. The level of knowledge and attitude were greater than 67% and 62 % respectively. Concerning source of information 418(51.7%) of them were obtained from medias about the important of blood donation. But there was a gap regarding age categories and sex who should be involved in blood donation the majority 311(38.4%) of them respond that male and female age of <18 years Old > 65 years. Practice of the study participants showed that too low 195(24.1%), of the respondents. The major reasons mentioned for not donating blood among non-donors were lack of information on where, when, and how to donate blood 366(45.2%), perception of not being fitted to donate blood 63 (7.8%), fear of HIV after blood donation 17(2.1%), and didn't have any blood to spare 29(3.6%). The knowledge of the society were not adequate. So this research finding recommend to create awareness to the society about blood donation and its advantage in the community through training, radio and template.