Acrylic-based high internal phase emulsion polymeric monolith for capillary electrochromatography

Tunc Y., Golgelioglu C., Hasirci N., ULUBAYRAM K., TUNCEL S. A.

JOURNAL OF CHROMATOGRAPHY A, cilt.1217, sa.10, ss.1654-1659, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1217 Sayı: 10
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.chroma.2010.01.020
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY A
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1654-1659
  • Anahtar Kelimeler: Monolithic columns, PolyHIPE monoliths, Open-cell structure, Capillary electrochromatography, FUSED-SILICA CAPILLARIES, STATIONARY PHASES, SEPARATION MEDIA, COLUMNS, METHACRYLATE, ELECTROPHORESIS, CHROMATOGRAPHY
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

The use of high internal phase emulsion polymers (polyHIPEs) for CEC applications has remained relatively Unexplored. A few reports exist in the literature for the preparation of similar structures. In this study, polyHIPEs having high porosity, and interconnected open-cell structure, were introduced and evaluated as stationary phase for CEC. The polyHIPE monolithic columns were prepared by the in situ polymerization of isodecylacrylate (IDA) and divinylbenzene (DVB) in the continuous phase of a high internal phase emulsion (HIPE). Due to its well-defined polyHIPE structure with interconnected micron size spherical voids, the columns synthesized with different initiator concentrations were Successfully used for the separation of alkylbenzenes. Furthermore, the columns indicated a strong electroosmotic flow (EOF) without any additional EOF generating monomer probably due to the presence of ionizable sulfate groups coming from the water-soluble initiator used in the preparation of polyHIPE matrix. The best chromatographic performance in the separation of alkylbenzenes was achieved by using 70% ACN in the mobile phase with high column efficiency (up to 200000 plates/m). (C) 2010 Elsevier B.V. All rights reserved.