Seamless and robust alginate/gelatin coating on Ti-6Al-4V as a gap filling interphase


PAZARÇEVİREN A. E. , Akbaba S., TEZCANER A. , KESKİN D. , EVİS Z.

APPLIED SURFACE SCIENCE, vol.581, 2022 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 581
  • Publication Date: 2022
  • Doi Number: 10.1016/j.apsusc.2021.152393
  • Title of Journal : APPLIED SURFACE SCIENCE
  • Keywords: Implant coating, Alginate, Gelatin, Hydrogel, Titanium, APTES, TITANIUM SURFACE, OSSEOINTEGRATION, FUNCTIONALIZATION, HYDROGEL, GELATIN, APTES, IMMOBILIZATION, PERFORMANCE, IMPLANTS, STRENGTH

Abstract

Durable, biocompatible and interphases composed of interpenetrating network of alginate and gelatin (A/G) with long shelf-life were produced. Titanium implants were conditioned with acid:peroxide followed by sodium hydroxide (NaOH) etching. Surfaces were grafted with 3-aminopropyl triethoxysilane (APTES) and optimized. It was hypothesized that interpenetrating network of A/G coated on Ti-APTES in the presence of 0.2 M sodium chloride could improve the adhesive strength and create a highly cohesive hydrogel interphase. Optimized A/G ratio and APTES grafting procedure allowed us to reproducibly produce a layer which resulted in a gap-filling, seamless and robust coating that can assume implant shape, maintain morphology and stay intact for a year at room temperature. Especially A/G 1:3 interphase coating (16.41 +/- 0.34 mu m) demonstrated good adhesion (1.28 +/- 0.32 N), water uptake (589% +/- 78), hydrolytic degradation (63 +/- 1%), low initial wettability in simulated body fluid, but demonstrated rapid methylene blue (MB) uptake (80% of drug within 1 h) and burst release (100% of MB within similar to 3 h), and favored osteoblast adhesion and proliferation over time. Furthermore, A/G 1:3 maintained integrity and showed minimal loss of strength over 12 months. Hence, A/G 1:3 interphase showed substantial potential to improve biofunctionality of bioinert implants.