Seamless and robust alginate/gelatin coating on Ti-6Al-4V as a gap filling interphase

PAZARÇEVİREN A. E., Akbaba S., TEZCANER A., KESKİN D., EVİS Z.

APPLIED SURFACE SCIENCE, cilt.581, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 581
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.apsusc.2021.152393
  • Dergi Adı: APPLIED SURFACE SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Implant coating, Alginate, Gelatin, Hydrogel, Titanium, APTES, TITANIUM SURFACE, OSSEOINTEGRATION, FUNCTIONALIZATION, HYDROGEL, GELATIN, APTES, IMMOBILIZATION, PERFORMANCE, IMPLANTS, STRENGTH
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Durable, biocompatible and interphases composed of interpenetrating network of alginate and gelatin (A/G) with long shelf-life were produced. Titanium implants were conditioned with acid:peroxide followed by sodium hydroxide (NaOH) etching. Surfaces were grafted with 3-aminopropyl triethoxysilane (APTES) and optimized. It was hypothesized that interpenetrating network of A/G coated on Ti-APTES in the presence of 0.2 M sodium chloride could improve the adhesive strength and create a highly cohesive hydrogel interphase. Optimized A/G ratio and APTES grafting procedure allowed us to reproducibly produce a layer which resulted in a gap-filling, seamless and robust coating that can assume implant shape, maintain morphology and stay intact for a year at room temperature. Especially A/G 1:3 interphase coating (16.41 +/- 0.34 mu m) demonstrated good adhesion (1.28 +/- 0.32 N), water uptake (589% +/- 78), hydrolytic degradation (63 +/- 1%), low initial wettability in simulated body fluid, but demonstrated rapid methylene blue (MB) uptake (80% of drug within 1 h) and burst release (100% of MB within similar to 3 h), and favored osteoblast adhesion and proliferation over time. Furthermore, A/G 1:3 maintained integrity and showed minimal loss of strength over 12 months. Hence, A/G 1:3 interphase showed substantial potential to improve biofunctionality of bioinert implants.