PLASMID PATTERNS OF BACILLUS-THURINGIENSIS 81 AND ITS CRYSTAL-NEGATIVE MUTANTS

AFKHAMI P., OZCENGIZ G., ALAEDDINOGLU N.

BIOTECHNOLOGY LETTERS, vol.15, no.12, pp.1247-1252, 1993 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 12
  • Publication Date: 1993
  • Doi Number: 10.1007/bf00130306
  • Journal Name: BIOTECHNOLOGY LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1247-1252
  • Middle East Technical University Affiliated: Yes

Abstract

A local isolate of Bacillus thuringiensis (B.t. 81) contained five distinct plasmids Relative amounts of these plasmids varied at different stages of growth.Two Cry- mutants of the strain maintained all plasmids. The mutants, however, exhibited some variations in the patterns of large plasmids depending on the composition of culture medium even though they failed to produce crystal protein. Restriction analysis of the plasmids was also carried out for future cloning studies.