Production and characterization of xylanases of a Bacillus strain isolated from soil

Avcioglu B., Eyupoglu B., Bakir U.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.21, no.1, pp.65-68, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1007/s11274-004-1934-9
  • Journal Name: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.65-68
  • Keywords: Bacillus, characterization, paper pulp, production, xylanase, ALKALINE XYLANASE, MICROORGANISMS, PURIFICATION
  • Middle East Technical University Affiliated: No

Abstract

Xylanase production was performed by growing a Bacillus isolate on agricultural by-products, wheat straw, wheat bran, corn cobs and cotton bagasse. A maximum xylanase activity of 180 U/ml was obtained together with a cellulase activity of 0.03 U/ml on 4 (w/v) corn cobs. Electrophoretic analysis showed the presence of three endo-beta-1, 4-xylanases having molecular weights of about 22, 23 and 40 kDa. Xylanolytic activity was stable up to 50degreesC in the pH range of 4.5-10 and the highest activity was observed at 70degreesC and pH 6.5.