Development of pH Sensitive Alginate/Gum Tragacanth Based Hydrogels for Oral Insulin Delivery


Cikrikci S. , Mert B. , Oztop M. H.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.66, pp.11784-11796, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 66
  • Publication Date: 2018
  • Doi Number: 10.1021/acs.jafc.8b02525
  • Title of Journal : JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Page Numbers: pp.11784-11796
  • Keywords: hydrogel, insulin release, NMR relaxometry, modeling, CORE-SHELL NANOPARTICLES, DRUG-DELIVERY, WHEY-PROTEIN, CONTROLLED-RELEASE, CHITOSAN NANOPARTICLES, PHYSICOCHEMICAL PROPERTIES, RHEOLOGICAL BEHAVIOR, SWELLING BEHAVIOR, NATURAL POLYMERS, IN-VITRO

Abstract

Insulin entrapped alginate-gum tragacanth (ALG-GT) hydrogels at different ALG replacement ratios (100, 75, 50, 25) were prepared through an ionotropic gelation method, followed by chitosan (CH) polyelectrolyte complexation. A mild gelation process without the use of harsh chemicals was proposed to improve insulin efficiency. Retention of almost the full amount of entrapped insulin in a simulated gastric environment and sustained insulin release in simulated intestinal buffer indicated the pH sensitivity of the gels. Insulin release from hydrogels with different formulations showed significant differences (p < 0.05). Time domain (TD) NMR relaxometry experiments also showed the differences for different formulations, and the presence of CH revealed that ALG-GT gel formulation could be used as an oral insulin carrier at optimum concentrations. The hydrogels formulated from biodegradable, biocompatible, and nontoxic natural polymers were seen as promising devices for potential oral insulin delivery.