© 2020 IEEE.Age of Information (AoI) has gained importance as a Key Performance Indicator (KPI) for characterizing the freshness of information in information-update systems and time-critical applications. Recent theoretical research on the topic has generated significant understanding of how various algorithms perform in terms of this metric on various system models and networking scenarios. In this paper, we study AoI behavior on experimental implementations of packet flows among IoT nodes and over the Internet. AoI measurements are taken for several transport protocols such as TCP, UDP and web-socket, on wired and wireless links. Practical issues such as synchronization and selection of hardware along with transport protocol, and their effects on AoI are discussed. The results provide insight toward application and transport layer mechanisms for optimizing AoI in real-life networks.