Parametric continuous feed stream design to fine-tune fed-batch bioreactor performance: recombinant human growth hormone production in Bacillus subtilis
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.91, sa.11, ss.2740-2750, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 91 Sayı: 11
- Basım Tarihi: 2016
- Doi Numarası: 10.1002/jctb.4864
- Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2740-2750
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
BACKGROUND: Design principles of a fed-batch bioreactor system to fine-tune bioreactor performance by increasing production and productivity are investigated. To enhance the production and productivity in fed-batch fermentation of recombinant human growth hormone (rhGH) by Bacillus subtilis, this work develops a continuous feed stream (CFS) design method with the parameters, cultivation time to switch batch-to fed-batch operation, so called transition time, t(T), and specific growth rate, mu.