Parametric continuous feed stream design to fine-tune fed-batch bioreactor performance: recombinant human growth hormone production in Bacillus subtilis

ÇALIK GARCİA G., KOCABAŞ P., Afsar H., ÇALIK P., Ozdamar T. H.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.91, no.11, pp.2740-2750, 2016 (SCI-Expanded) identifier identifier


BACKGROUND: Design principles of a fed-batch bioreactor system to fine-tune bioreactor performance by increasing production and productivity are investigated. To enhance the production and productivity in fed-batch fermentation of recombinant human growth hormone (rhGH) by Bacillus subtilis, this work develops a continuous feed stream (CFS) design method with the parameters, cultivation time to switch batch-to fed-batch operation, so called transition time, t(T), and specific growth rate, mu.