In a previous ultrastructural study, the benefit of a single high dose of vitamin D-3 On fracture healing in a healthy animal model was demonstrated. This study examined the biomechanical consequences of applying a single high dose of vitamin D-3 in a healthy rabbit model subsequent to femoral fracture. The fracture load, the values of energy absorbed until fracture and the flexural rigidity values of the vitamin D group were significantly higher than the corresponding ones of the control group in the case of fracture. On the other hand, for intact bones, those values did not differ significantly between the two groups. It was concluded that single high-dose vitamin D-3 application had positive effects on fracture healing in a healthy animal model, as far as the parameters related to mechanical strength are concerned.