Proteomic Analysis of a hom-Disrupted, Cephamycin C Overproducing Streptomyces clavuligerus

Unsaldi E., KURT KIZILDOĞAN A., ÖZCAN S., Becher D., Voigt B., Aktas C., ...More

PROTEIN AND PEPTIDE LETTERS, vol.28, no.2, pp.205-220, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.2174/0929866527666200723163655
  • Journal Name: PROTEIN AND PEPTIDE LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE
  • Page Numbers: pp.205-220
  • Keywords: Cephamycin C, Streptomyces clavuligerus, hom-disruption, proteomics, MALDI-TOF/MS, nanoLC-MS/MS, CLAVULANIC ACID PRODUCTION, SECONDARY METABOLISM, HETEROLOGOUS EXPRESSION, ANTIBIOTIC PRODUCTION, BIOSYNTHETIC GENES, DEHYDROGENASE GENE, CRYSTAL-STRUCTURE, TETR FAMILY, SYSTEM, COELICOLOR
  • Middle East Technical University Affiliated: Yes

Abstract

Background: Streptomyces clavuligerus is prolific producer of cephamycin C, a medically important antibiotic. In our former study, cephamycin C titer was 2-fold improved by disrupting homoserine dehydrogenase (hom) gene of aspartate pahway in Streptomyces clavuligerus NR-RL 3585.