A Revertible, Autonomous, Self-Assembled DNA-Origami Nanoactuator

MARİNİ M., Piantanida L., Musetti R., Bek A., Dong M., Besenbacher F., ...Daha Fazla

NANO LETTERS, cilt.11, sa.12, ss.5449-5454, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 12
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1021/nl203217m
  • Dergi Adı: NANO LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5449-5454
  • Anahtar Kelimeler: DNA origami, self-assembly, autonomous switch, reusable, actuation, FOLDING DNA, DESIGN, SHAPES
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

A DNA-origami actuator capable of autonomous internal motion in accord to an external chemical signal was designed, built, operated and imaged. The functional DNA nanostructure consists of a disk connected to an external ring in two, diametrically opposite points. A single stranded DNA, named probe, was connected to two edges of the disk perpendicularly to the axis of constrain. In the presence of a hybridizing target molecule, the probe coiled into a double helix that stretched the inner disk forcing the edges to move toward each other. The addition of a third single stranded molecule that displaced the target from the probe restored the initial state of the origami. Operation, dimension and shape were carefully characterized by combining microscopy and fluorescence techniques.