INFLUENCE OF MEMBRANE-COMPONENTS ON THE STABILITY AND DRUG-RELEASE PROPERTIES OF REVERSE-PHASE EVAPORATION VESICLES (REVS) - LIGHT-SENSITIVE ALL-TRANS RETINAL, NEGATIVELY CHARGED PHOSPHOLIPID DICETYLPHOSPHATE AND CHOLESTEROL

GURSEL M., HASIRCI V.

JOURNAL OF MICROENCAPSULATION, vol.12, no.6, pp.661-669, 1995 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 6
  • Publication Date: 1995
  • Doi Number: 10.3109/02652049509006796
  • Journal Name: JOURNAL OF MICROENCAPSULATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.661-669
  • Middle East Technical University Affiliated: Yes

Abstract

Incorporation of a negatively charged phospholipid, dicetylphosphate, initially increased encapsulation efficiency (from 12 to 24%) but beyond 5% (molar) a detrimental effect was observed. Rate of drug release from REVs was, for most cases, found to be bi-phasic implying partitioning between the lipid bilayer and the aqueous compartment. It was not possible to prepare liposomes with more than 1% (molar) all-trans retinal (ATR) as a membrane component. When ATR was reduced to 0.5% (molar), encapsulation efficiency increased to 7.76%. Upon exposure to long wave UV (365 nm), release from ATR containing REVs was increased and this was attributed to the formation of 13-cis isomer as indicated by HPLC and UV spectroscopy data.