Transformation of lentil (Lens culinaris M.) cotyledonary nodes by vacuum infiltration of Agrobacterium tumefaciens

Mahmoudian M., Yucel M., Oktem H.

PLANT MOLECULAR BIOLOGY REPORTER, vol.20, no.3, pp.251-257, 2002 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 3
  • Publication Date: 2002
  • Doi Number: 10.1007/bf02782460
  • Journal Name: PLANT MOLECULAR BIOLOGY REPORTER
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.251-257
  • Keywords: Agrobacterium, cotyledonary node, GUS, Lens culinaris, lentil, vacuum infiltration, TRANSGENIC PLANTS, GENE-EXPRESSION, L., SYSTEM, MEDIK
  • Middle East Technical University Affiliated: Yes

Abstract

Lentil cotyledonary nodes are some of the most regenerative tissues in legumes. Attempts to transform them by vacuum filtration have been Limitedly successful. This report describes a rapid and convenient transient expression protocol based on vacuum infiltration of Agrobacterium cells into lentil cotyledonary nodes. Vacuum-infiltrated tissues had significantly (P < .05) higher transient GUS expression than did noninfiltrated tissues. Under optimal conditions (infiltration at 200 mmHg for 20 min), 95% of the Agrobacterium-infiltrated explants exhibited an average of 16 blue foci. We believe this to be the first report of this technique for transient gene expression in lentil cotyledonary nodes.