Flow injection fluorescence determination of dopamine using a photo induced electron transfer (PET) boronic acid derivative


Seckin Z., Volkan M.

ANALYTICA CHIMICA ACTA, cilt.547, sa.1, ss.104-108, 2005 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 547 Konu: 1
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.aca.2005.02.037
  • Dergi Adı: ANALYTICA CHIMICA ACTA
  • Sayfa Sayıları: ss.104-108

Özet

An automated flow injection analysis system was developed for the fluorometric determination of dopamine in pharmaceutical injections. The method is based on the quenching effect of dopamine on m-dansylaminophenyl boronic acid (DAPB) fluorescence due to the reverse photo induced electron transfer (PET) mechanism. Effects of pH and interfering species on the determination of dopamine were examined. Calibration for dopamine, based on quenching data, was linear in the concentration range of 1.0 x 10(-5) to 1.0 x 10(-4) M. Detection limit (3s) of the method was found to be 3.7 x 10(-6) M. Relative standard deviation of 1.2% (n = 10) was obtained with 1.0 x 10(-5) M dopamine standard solution. The proposed method was applied successfully for the determination of dopamine in pharmaceutical injection sample. The sampling rate was determined as 24 samples per hour. (c) 2005 Elsevier B.V. All rights reserved.