Production of recombinant human erythropoietin from Pichia pastoris and its structural analysis

Celik E., Calik P., Halloran S. M., Oliver S. G.

JOURNAL OF APPLIED MICROBIOLOGY, cilt.103, sa.6, ss.2084-2094, 2007 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 103 Sayı: 6
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1111/j.1365-2672.2007.03448.x
  • Dergi Adı: JOURNAL OF APPLIED MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2084-2094
  • Anahtar Kelimeler: AOX1 promoter, erythropoietin, expression, factor Xa, glycan, his-tag, MALDI, Pichia pastoris, ASSISTED-LASER-DESORPTION/IONIZATION, TIME-OF-FLIGHT, MASS-SPECTROMETRY, DESORPTION IONIZATION, PROTEIN EXPRESSION, LINKED GLYCANS, YEAST, OLIGOSACCHARIDES, GLYCOPROTEIN, DEGLYCOSYLATION
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Aims: To design and investigate a recombinant expression system producing a therapeutically important glycoprotein, human erythropoietin (rHuEPO), by Pichia pastoris.