Systems biology based miRNA–mRNA expression pattern analysis of Emodin in breast cancer cell lines

Sakalli-Tecim E., GÜR DEDEOĞLU B., GÜRAY N. T.

Pathology Research and Practice, vol.249, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 249
  • Publication Date: 2023
  • Doi Number: 10.1016/j.prp.2023.154780
  • Journal Name: Pathology Research and Practice
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS
  • Keywords: Breast cancer, Emodin, Estrogen receptor, miRNA, Phytoestrogen, Systems biology
  • Middle East Technical University Affiliated: Yes

Abstract

Breast cancer has been among the most prominent cancers with high mortality. Currently most of the offered therapeutics are toxic; hence, less toxic therapeutic intervention is required. Here, we studied the molecular mechanisms of the effect of a phytoestrogen Emodin on estrogen receptor positive MCF-7 and negative MDA-MB-231 cells by carrying out a comprehensive network assessment. Differentially expressed microRNAs along with their previously identified differentially expressed mRNAs were analyzed through microarrays by using integrative systems biology approach. For each cell line miRNA-target gene networks were built, gene ontology and pathway enrichment analyses were performed, enrichment maps were constructed and the potential key genes, miRNAs and miRNA-gene interactions were studied.