GPCR-G alpha protein precoupling: Interaction between Ste2p, a yeast GPCR, and Gpalp, its G alpha protein, is formed before ligand binding via the Ste2p C-terminal domain and the Gpalp N-terminal domain


Cevheroglu O., Becker J. M. , Son Ç. D.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, vol.1859, pp.2435-2446, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1859
  • Publication Date: 2017
  • Doi Number: 10.1016/j.bbamem.2017.09.022
  • Journal Name: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2435-2446
  • Keywords: G protein-coupled receptor (GPCR), Receptor signaling, Bioluminescence resonance energy transfer (BRET), Bimolecular fluorescence complementation (BiFC), GPCR-G protein heterodimerization, LIGHT-ACTIVATED RHODOPSIN, HETEROTRIMERIC G-PROTEINS, COVALENT CROSS-LINKING, SACCHAROMYCES-CEREVISIAE, FACTOR RECEPTOR, SIGNAL-TRANSDUCTION, PHEROMONE RECEPTOR, LIVING CELLS, COUPLED RECEPTORS, FLUORESCENCE COMPLEMENTATION
  • Middle East Technical University Affiliated: Yes

Abstract

G protein coupled receptors bind ligands that initiate intracellular signaling cascades via heterotrimeric G proteins. In this study, involvement of the N-terminal residues of yeast 9-alpha (Gpalp) with the C-terminal residues of a full-length or C-terminally truncated Ste2p were investigated using bioluminescence resonance energy transfer (BRET), a non-radiative energy transfer phenomenon where protein-protein interactions can be quantified between a donor bioluminescent molecule and a suitable acceptor fluorophore. Constitutive and position-dependent BRET signal was observed in the absence of agonist (alpha-factor). Upon the activation of the receptors with alpha-factor, no significant change in BRET signal was observed. The location of Ste2p-Gpalp heterodimer was investigated using confocal fluorescence microscopy and bimolecular fluorescence cornplementation (BiFC) assay, a technique where two non-fluorescent fragments of a fluorescent protein reassemble in vivo to restore fluorescence property thereby directly reporting a protein-protein interaction. BiFC experiments resulted in a dimerization signal intracellularly during biosynthesis on the endoplasmic reticulum (ER) and on the plasma membrane (PM). The constitutive BRET and BiFC signals observed on ER between Ste2p and Gpalp in their quiescent and activated states are indicative of pre-coupling between these two proteins.