Akademik Veri Yönetim Sistemi
Pakistan Journal of Biological Sciences, cilt.9, sa.14, ss.2579-2585, 2006 (Scopus)
Thermal tolerances and heat shock protein (s) [HSP(s)] of wild wheat species (Aegilops biuncialis Vis.) and Triticum durum Desf. cv. Harran-95 were investigated in etiolated seedlings. The thermal tolerances of the genotypes were determined by growth experiment and cell viability test. The HSPs were separated by two-dimensional (2-D) gel electrophoresis technique. In all the experiments, the two-day old etiolated seedlings were exposed to control (23°C, 24 h), acclimation (37°C, 24 h) and heat stress (37°C, 24 h→50°C, 1 h) temperature treatments. In growth experiment, there was no significant difference between the values of acquired thermal tolerance (%) of Ae. biuncialis and cv. Harran-95 in all treatments. In cell viability test, the values of acquired thermal tolerance (%) for Ae. biuncialis and cv. Harran-95 were detected as 54.5 and 28.4%, respectively. The molecular weights of HSPs synthesized in coleoptile tissues ranged from 19.7 kDa to 67.5 kDa and pi values ranged from 6.3 to 7.3. Most of the HSPs synthesized at acclimation treatment disappeared at heat stress treatment in Ae. biuncialis. Four newly synthesized HSPs with molecular weights of 19.7, 19.7, 25.9 and 26.6 kDa (pI values of 6.6, 6.5, 6.6 and 6.4, respectively) were common between Ae. biuncialis and cv. Harran-95 at both acclimation and heat stress treatments compared to control. © 2006 Asian Network for Scientific Information.