The role of bcsE gene in the pathogenicity of Salmonella

Ozdemir C., AKÇELİK N., ÖZDEMİR F. N., Evcili I., Kahraman T., GÜRSEL İ., ...More

PATHOGENS AND DISEASE, vol.79, no.6, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 79 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1093/femspd/ftab037
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE
  • Keywords: Salmonella, bcsE, biofilm, invasion, persistence, pathogenicity
  • Middle East Technical University Affiliated: No


The effects of the bcsE gene and BcsE protein on bacterial physiology and pathogenicity in SalmonellaTyphimurium and Salmonella Group C1 were investigated. It was observed that biofilm and pellicle formation did not occur in the bcsE gene mutants of wild-type strains. Besides, the 'rdar' (red, dry, rough) biofilm morphotype in wild-type strains changed significantly in the mutants. In terms of the bcsE gene, the swimming and swarming motility in mutant strains showed a dramatic increase compared to the wild-type strains. The Salmonella bcsE gene was cloned into Escherichia coli BL21, and the his-tagged protein produced in this strain was purified to obtain polyclonal antibodies in BALB/c mice. The antibodies were showed labeled antigen specificity to the BscE protein. As a result of immunization and systemic persistence tests carried out with BALB/c mice, BscE protein was determined to trigger high levels of humoral and cellular responses (Th1 cytokine production, IgG2a/IgG1 > 1). Systemic persistence in the liver and spleen samples decreased by 99.99% and 100% in the bcsE mutant strains. Finally, invasion abilities on HT-29 epithelial cells of wild-type strains were utterly disappeared in their bcsE gene mutant strains.This article describes the role of BcsE, which is a c-di-GMP binding protein, in Salmonella biofilm formation properties and pathogenicity.