In this study, a novel virtual reality-based interactive method combined with the application of a graphical processing unit (GPU) is proposed for the semiautomatic segmentation of 3D magnetic resonance imaging (MRI) of the brain. The key point of our approach is to use haptic force feedback guidance for the selection of seed points in a bounded volume with similar intensity and gradient. For the automatic determination of a bounded volume of segmentation in real time, parallel computation on the GPU is used. Automatic segmentation is applied in this adjustable bounded spherical volume with a variable diameter, which is controlled according to the edge map acquired from the gradient map. The haptic force feedback is used in order to guide the user to remain in a volume, where the intensity and gradient change are under a defined threshold range. After each seed point selection, the segmentation algorithm works inside the bounded volume of the ball with an adjusted diameter. The proposed segmentation method based on force and visual feedback with the advantage of adjustable bounded volume is not only accurate and effective in narrow spaces near the boundaries of different layers, but also fast in large homogeneous spaces since the radius of the ball increases in such regions. Parallel programming on the GPU is used for computing gradient change in selected directions, which is needed for the self-adjustment of the sphere diameter. Gradient values are used for calculating the haptic force on the CPU in real time. In this study, two haptic devices are used, one for getting haptic force feedback and the other for camera guidance during 3D visualization. A comparison between manual segmentation of MRI by an expert surgeon and the proposed segmentation algorithm is done. The proposed segmentation procedure is completed 4 times faster than the manual segmentation with similar accuracy.